Product Detail

High-Performance Liquid Chromatography (HPLC) is utilized in a wide range of fields, such as medicine, food production, chemistry, and environmental science, making the demand for HPLC systems higher among the various types of analytical equipment. The Hitachi Primaide HPLC is designed for long-term, stable operation, and features both high reliability and superior durability. It offers tremendous capability for everyday analysis. Features Simple operation for any HPLC user Even in small to mid-scale facilities, individual operators see increasing opportunities to use multiple HPLC systems. Thanks to its simple design and front-access layout, maintenance of the Primaide is easy. Even a beginner at LC analysis can confidently operate the Primaide. Excellent basic performance The demand for HPLC systems with higher precision, better reproducibility, and improved stability is increasing. The Primaide is built according to strict specifications to meet such stringent requirements. In addition, labs can apply the Primaide to an even wider range of applications by employing optional units, such as a sophisticated DAD (Diode Array Detector) and an autosampler cooling unit. Reliable durability Hitachi HPLC systems have earned a reputation as highly durable machines, and the Primaide is no exception. Our strict production quality standards ensure both high performance and endurance. These standards are applied to all equipment from systems to accessories. System Features Optional units mounted inside provide clean appearance All principal options, including a degasser, gradient unit, and sample cooling system can be mounted inside the main body. It is not necessary to make room for optional units. Front access layout for improved operation and maintenance Every module is accessed and handled from the front. Since connections are arranged on the front, it is easy to replace columns and manipulate the flow system. For easier maintenance, the lamp and cell unit are also easily accessible. Leak sensors in all modules Any leakage in the flow system is detected by leak sensors built into each module. After detection the system halts, ensuring improved safety and reliability. Complete control with system manager A control panel is not necessary, as everything can be controlled via the system manager. By installing a UI- pad (optional), each module can be controlled separately. e-Line and USB The e-Line is an advanced version of the existing D-Line. All modules are connected by an e-Line in a single chain, and all the communications are integrated. The use of digital signals improves the reliability of data transactions. The interface between the PC and equipment is via versatile USB. A wide range of PCs are supported, enabling flexible configuration. GLP function A Hg lamp is installed in the detector, and wavelengths in the UV range can be calibrated using its emission line (254 nm) (installed in the Primaide 1410 UV / 1430 DAD). Each module stores various types of quality assurance information, for improved data reliability. Module lineup: 1110 Pump The Primaide 1110 Pump has been developed for reliable analysis and shares characteristics with Hitachi's existing HPLC pump models. Its consistently high level of performance provides users with strong support in everyday analysis. Improvement of flow rate stability For pulse compensation, a proprietary real-time feedback method has been developed for Hitachi’s pumps. Not only has the pulse been significantly reduced, but the Primaide 1110 Pump estimates pressure fluctuations and employs the optimal timing to set the plunger speed interval. Improvement of retention time reproducibility For gradient analysis, injection by the Primaide 1210 Autosampler is synchronized with the movements of the pump. This ensures a highly reproducible retention time, and precise analysis. Gradient Performance Evaluation/RT and Area Reproducibility Peak No. Component Retention Time Average / min. R.S.D. / % 1 Acetanilide 2.032 0.076 2 Acetophenone 2.793 0.080 3 Propiophenone 3.581 0.096 4 Butyrophenone 4.495 0.102 5 Benzophenone 4.809 0.098 6 Valerophenone 5.639 0.083 7 Hexanophenone 6.975 0.082 8 Heptanophenone 8.413 0.068 9 Octanophenone 9.882 0.078 Condition Column HITACHI LaChrom C18 (5 µm) 4.6 mm I.D.×150 mm L. Mobile phase A : CH3OH B : H2O Gradient program 0 - 11 min A 65 → 100% Column Temperature 40°C Flow rate 1.0 mL/min Detection UV 265 nm Injection vol. 10 µL (100 g/mL each.) 1210 Autosampler The Primaide 1210 Autosampler incorporates direct injection, allowing the automation of consecutive analyses without wasting precious sample. Direct injection of small-quantity samples and quicker analysis In the direct injection method, the sampling needle becomes part of the flow channel so that the sample in the needle flows into the channel with zero waste. This is a great advantage in the analysis of valuable small-quantity samples. In addition, since the injection method is simpler than using a sampling loop, the injection cycle is shortened by half. This method accelerates throughput and improves efficiency in the analysis of many samples. Improved reproducibility results in high-precision analysis The syringe for sample measurement is driven by a high-performance motor. The drive improves the reproducibility of injection. Furthermore, peak retention times are also more repeatable due to synchronization of injection with the pump motion, achieving higher analytical reliability. Injection Volume Reproducibility